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cryo cd45 goat r d systems  (R&D Systems)


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    R&D Systems cryo cd45 goat r d systems
    Figure 3. Cell proliferation is increased at 6 h following aortic valve (AoV) endothelial injury. A, Phospho-histone H3 (pHH3) immunoreactivity in sham (i–iii) and injured (iv–vi) AoVs from 3 mo old mice at 6 h (I and iv), 48 h (ii and v), and 4 wk (iii and vi) postinjury. Arrows indicate immunoreactive cells. B, Quantification of data shown in A as a percent of pHH3-positive cells over the total number of DAPI-labeled nuclei per valve. Six hour injury (n=7) vs 6 h sham (n=5) *P=0.0005, 48 h injury (n=6) vs 48 h sham (n=5) #P=0002, 48 h injury (n=6) vs 6 h injury (n=7) P=0.0009, 2-tailed unpaired Student t test. C, pHH3 and VE-cadherin (vascular endothelial cadherin) double-stained immunofluorescence in injured AoVs at 6 h (i) and 48 h (ii) postsurgery. White arrowheads indicate double-positive cells within the interstitium; arrowheads indicate double-positive cells within the endothelial compartment. D, i and ii, Quantification of data shown in C. n=6 per group, not significant, 2-tailed unpaired Student t test. E, Quantification of VE- cadherin–positive cells as a percentage of the total number of DAPI labeled at nuclei per valve at indicated time points. n=6 per group, not significant, 2-tailed unpaired Student t test. F, pHH3 and <t>CD45</t> double-stained immunofluorescence in injured AoVs at 6 h (i) and 48 h (ii) postsurgery. White arrowheads indicate double-positive cells within the interstitium, arrowheads indicate double-positive cells within the endothelial compartment. G, Quantification of data shown in F. n=6 per group, not significant, 2-tailed unpaired Student t test. H, Quantification of <t>CD45-positive</t> cells as a percentage of the total number of DAPI (4′,6-diamidino-2-phenylindole) labeled at nuclei per valve at indicated time points. n=6, not significant, 2-tailed unpaired Student t test.
    Cryo Cd45 Goat R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 159 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cryo+cd45+goat+r+d+systems/Mouse+CD45+Antibody/10__1161_slash_atvbaha__121__316450-161-41-44
    Average 96 stars, based on 159 article reviews
    cryo cd45 goat r d systems - by Bioz Stars, 2026-09
    96/100 stars

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    1) Product Images from "Tgfβ1-Cthrc1 Signaling Plays an Important Role in the Short-Term Reparative Response to Heart Valve Endothelial Injury"

    Article Title: Tgfβ1-Cthrc1 Signaling Plays an Important Role in the Short-Term Reparative Response to Heart Valve Endothelial Injury

    Journal: Arteriosclerosis, Thrombosis, and Vascular Biology

    doi: 10.1161/atvbaha.121.316450

    Figure 3. Cell proliferation is increased at 6 h following aortic valve (AoV) endothelial injury. A, Phospho-histone H3 (pHH3) immunoreactivity in sham (i–iii) and injured (iv–vi) AoVs from 3 mo old mice at 6 h (I and iv), 48 h (ii and v), and 4 wk (iii and vi) postinjury. Arrows indicate immunoreactive cells. B, Quantification of data shown in A as a percent of pHH3-positive cells over the total number of DAPI-labeled nuclei per valve. Six hour injury (n=7) vs 6 h sham (n=5) *P=0.0005, 48 h injury (n=6) vs 48 h sham (n=5) #P=0002, 48 h injury (n=6) vs 6 h injury (n=7) P=0.0009, 2-tailed unpaired Student t test. C, pHH3 and VE-cadherin (vascular endothelial cadherin) double-stained immunofluorescence in injured AoVs at 6 h (i) and 48 h (ii) postsurgery. White arrowheads indicate double-positive cells within the interstitium; arrowheads indicate double-positive cells within the endothelial compartment. D, i and ii, Quantification of data shown in C. n=6 per group, not significant, 2-tailed unpaired Student t test. E, Quantification of VE- cadherin–positive cells as a percentage of the total number of DAPI labeled at nuclei per valve at indicated time points. n=6 per group, not significant, 2-tailed unpaired Student t test. F, pHH3 and CD45 double-stained immunofluorescence in injured AoVs at 6 h (i) and 48 h (ii) postsurgery. White arrowheads indicate double-positive cells within the interstitium, arrowheads indicate double-positive cells within the endothelial compartment. G, Quantification of data shown in F. n=6 per group, not significant, 2-tailed unpaired Student t test. H, Quantification of CD45-positive cells as a percentage of the total number of DAPI (4′,6-diamidino-2-phenylindole) labeled at nuclei per valve at indicated time points. n=6, not significant, 2-tailed unpaired Student t test.
    Figure Legend Snippet: Figure 3. Cell proliferation is increased at 6 h following aortic valve (AoV) endothelial injury. A, Phospho-histone H3 (pHH3) immunoreactivity in sham (i–iii) and injured (iv–vi) AoVs from 3 mo old mice at 6 h (I and iv), 48 h (ii and v), and 4 wk (iii and vi) postinjury. Arrows indicate immunoreactive cells. B, Quantification of data shown in A as a percent of pHH3-positive cells over the total number of DAPI-labeled nuclei per valve. Six hour injury (n=7) vs 6 h sham (n=5) *P=0.0005, 48 h injury (n=6) vs 48 h sham (n=5) #P=0002, 48 h injury (n=6) vs 6 h injury (n=7) P=0.0009, 2-tailed unpaired Student t test. C, pHH3 and VE-cadherin (vascular endothelial cadherin) double-stained immunofluorescence in injured AoVs at 6 h (i) and 48 h (ii) postsurgery. White arrowheads indicate double-positive cells within the interstitium; arrowheads indicate double-positive cells within the endothelial compartment. D, i and ii, Quantification of data shown in C. n=6 per group, not significant, 2-tailed unpaired Student t test. E, Quantification of VE- cadherin–positive cells as a percentage of the total number of DAPI labeled at nuclei per valve at indicated time points. n=6 per group, not significant, 2-tailed unpaired Student t test. F, pHH3 and CD45 double-stained immunofluorescence in injured AoVs at 6 h (i) and 48 h (ii) postsurgery. White arrowheads indicate double-positive cells within the interstitium, arrowheads indicate double-positive cells within the endothelial compartment. G, Quantification of data shown in F. n=6 per group, not significant, 2-tailed unpaired Student t test. H, Quantification of CD45-positive cells as a percentage of the total number of DAPI (4′,6-diamidino-2-phenylindole) labeled at nuclei per valve at indicated time points. n=6, not significant, 2-tailed unpaired Student t test.

    Techniques Used: Labeling, Staining, Immunofluorescence

    Related Articles

    Formalin-fixed Paraffin-Embedded:

    Article Title: Tgfβ1-Cthrc1 Signaling Plays an Important Role in the Short-Term Reparative Response to Heart Valve Endothelial Injury
    Article Snippet: .. Antibodies Antibody (α) Raised in Company, product number Dilution Section type Versican V0, V1 Rabbit Invitrogen no. PA1-1748A 1:200 Cryo Phospho-histone H3 Rabbit Sigma-Aldrich no. 06-570 1:200 Cryo pSmad2 Rabbit ThermoFisher Scientific 1:200 N/A VE-cadherin Goat R&D Systems no. AF1002 1:100 Cryo CD45 Goat R&D Systems no. AF114 1:100 Cryo Cthrc1 Rabbit MMCRI no. Vli55 1:100 FFPE Tgfβ1 Rabbit Novus Biologicals no. NBP1-80289 1:100 FFPE F4/80 Rat BioRad MCA497GA 1:400 FFPE Cryo indicates cryostat frozen sections; Cthrc1, collagen triple helix repeat containing 1; FFPE, formalin fixed, paraffin embedded; N/A, not applicable; pSmad2, phosphorylated Smad2; Tgfβ1, transforming growth factor beta 1; and VE-cadherin, vascular endothelial cadherin. ..



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    cryo cd45 goat r d systems  (R&D Systems)
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    R&D Systems cryo cd45 goat r d systems
    Figure 3. Cell proliferation is increased at 6 h following aortic valve (AoV) endothelial injury. A, Phospho-histone H3 (pHH3) immunoreactivity in sham (i–iii) and injured (iv–vi) AoVs from 3 mo old mice at 6 h (I and iv), 48 h (ii and v), and 4 wk (iii and vi) postinjury. Arrows indicate immunoreactive cells. B, Quantification of data shown in A as a percent of pHH3-positive cells over the total number of DAPI-labeled nuclei per valve. Six hour injury (n=7) vs 6 h sham (n=5) *P=0.0005, 48 h injury (n=6) vs 48 h sham (n=5) #P=0002, 48 h injury (n=6) vs 6 h injury (n=7) P=0.0009, 2-tailed unpaired Student t test. C, pHH3 and VE-cadherin (vascular endothelial cadherin) double-stained immunofluorescence in injured AoVs at 6 h (i) and 48 h (ii) postsurgery. White arrowheads indicate double-positive cells within the interstitium; arrowheads indicate double-positive cells within the endothelial compartment. D, i and ii, Quantification of data shown in C. n=6 per group, not significant, 2-tailed unpaired Student t test. E, Quantification of VE- cadherin–positive cells as a percentage of the total number of DAPI labeled at nuclei per valve at indicated time points. n=6 per group, not significant, 2-tailed unpaired Student t test. F, pHH3 and <t>CD45</t> double-stained immunofluorescence in injured AoVs at 6 h (i) and 48 h (ii) postsurgery. White arrowheads indicate double-positive cells within the interstitium, arrowheads indicate double-positive cells within the endothelial compartment. G, Quantification of data shown in F. n=6 per group, not significant, 2-tailed unpaired Student t test. H, Quantification of <t>CD45-positive</t> cells as a percentage of the total number of DAPI (4′,6-diamidino-2-phenylindole) labeled at nuclei per valve at indicated time points. n=6, not significant, 2-tailed unpaired Student t test.
    Cryo Cd45 Goat R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cryo+cd45+goat+r+d+systems/Mouse+CD45+Antibody/10__1161_slash_atvbaha__121__316450-161-41-44
    Average 96 stars, based on 1 article reviews
    cryo cd45 goat r d systems - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 3. Cell proliferation is increased at 6 h following aortic valve (AoV) endothelial injury. A, Phospho-histone H3 (pHH3) immunoreactivity in sham (i–iii) and injured (iv–vi) AoVs from 3 mo old mice at 6 h (I and iv), 48 h (ii and v), and 4 wk (iii and vi) postinjury. Arrows indicate immunoreactive cells. B, Quantification of data shown in A as a percent of pHH3-positive cells over the total number of DAPI-labeled nuclei per valve. Six hour injury (n=7) vs 6 h sham (n=5) *P=0.0005, 48 h injury (n=6) vs 48 h sham (n=5) #P=0002, 48 h injury (n=6) vs 6 h injury (n=7) P=0.0009, 2-tailed unpaired Student t test. C, pHH3 and VE-cadherin (vascular endothelial cadherin) double-stained immunofluorescence in injured AoVs at 6 h (i) and 48 h (ii) postsurgery. White arrowheads indicate double-positive cells within the interstitium; arrowheads indicate double-positive cells within the endothelial compartment. D, i and ii, Quantification of data shown in C. n=6 per group, not significant, 2-tailed unpaired Student t test. E, Quantification of VE- cadherin–positive cells as a percentage of the total number of DAPI labeled at nuclei per valve at indicated time points. n=6 per group, not significant, 2-tailed unpaired Student t test. F, pHH3 and CD45 double-stained immunofluorescence in injured AoVs at 6 h (i) and 48 h (ii) postsurgery. White arrowheads indicate double-positive cells within the interstitium, arrowheads indicate double-positive cells within the endothelial compartment. G, Quantification of data shown in F. n=6 per group, not significant, 2-tailed unpaired Student t test. H, Quantification of CD45-positive cells as a percentage of the total number of DAPI (4′,6-diamidino-2-phenylindole) labeled at nuclei per valve at indicated time points. n=6, not significant, 2-tailed unpaired Student t test.

    Journal: Arteriosclerosis, Thrombosis, and Vascular Biology

    Article Title: Tgfβ1-Cthrc1 Signaling Plays an Important Role in the Short-Term Reparative Response to Heart Valve Endothelial Injury

    doi: 10.1161/atvbaha.121.316450

    Figure Lengend Snippet: Figure 3. Cell proliferation is increased at 6 h following aortic valve (AoV) endothelial injury. A, Phospho-histone H3 (pHH3) immunoreactivity in sham (i–iii) and injured (iv–vi) AoVs from 3 mo old mice at 6 h (I and iv), 48 h (ii and v), and 4 wk (iii and vi) postinjury. Arrows indicate immunoreactive cells. B, Quantification of data shown in A as a percent of pHH3-positive cells over the total number of DAPI-labeled nuclei per valve. Six hour injury (n=7) vs 6 h sham (n=5) *P=0.0005, 48 h injury (n=6) vs 48 h sham (n=5) #P=0002, 48 h injury (n=6) vs 6 h injury (n=7) P=0.0009, 2-tailed unpaired Student t test. C, pHH3 and VE-cadherin (vascular endothelial cadherin) double-stained immunofluorescence in injured AoVs at 6 h (i) and 48 h (ii) postsurgery. White arrowheads indicate double-positive cells within the interstitium; arrowheads indicate double-positive cells within the endothelial compartment. D, i and ii, Quantification of data shown in C. n=6 per group, not significant, 2-tailed unpaired Student t test. E, Quantification of VE- cadherin–positive cells as a percentage of the total number of DAPI labeled at nuclei per valve at indicated time points. n=6 per group, not significant, 2-tailed unpaired Student t test. F, pHH3 and CD45 double-stained immunofluorescence in injured AoVs at 6 h (i) and 48 h (ii) postsurgery. White arrowheads indicate double-positive cells within the interstitium, arrowheads indicate double-positive cells within the endothelial compartment. G, Quantification of data shown in F. n=6 per group, not significant, 2-tailed unpaired Student t test. H, Quantification of CD45-positive cells as a percentage of the total number of DAPI (4′,6-diamidino-2-phenylindole) labeled at nuclei per valve at indicated time points. n=6, not significant, 2-tailed unpaired Student t test.

    Article Snippet: Antibodies Antibody (α) Raised in Company, product number Dilution Section type Versican V0, V1 Rabbit Invitrogen no. PA1-1748A 1:200 Cryo Phospho-histone H3 Rabbit Sigma-Aldrich no. 06-570 1:200 Cryo pSmad2 Rabbit ThermoFisher Scientific 1:200 N/A VE-cadherin Goat R&D Systems no. AF1002 1:100 Cryo CD45 Goat R&D Systems no. AF114 1:100 Cryo Cthrc1 Rabbit MMCRI no. Vli55 1:100 FFPE Tgfβ1 Rabbit Novus Biologicals no. NBP1-80289 1:100 FFPE F4/80 Rat BioRad MCA497GA 1:400 FFPE Cryo indicates cryostat frozen sections; Cthrc1, collagen triple helix repeat containing 1; FFPE, formalin fixed, paraffin embedded; N/A, not applicable; pSmad2, phosphorylated Smad2; Tgfβ1, transforming growth factor beta 1; and VE-cadherin, vascular endothelial cadherin.

    Techniques: Labeling, Staining, Immunofluorescence